CC-2 ac6 mt-

From W.T. Ebersold, UCLA, to Boynton-Gillham laboratory, Duke University, 1973

Phenotype: requires acetate for growth at high pH

The ac6 mutant was isolated by Eversole, and originally designated EV 6a. Eversole described his stock as a multigene mutant that frequently had more than two flagella per cell, but we have not observed this phenotype. It is likely that the stock received from Ebersold was the product of a cross rather than the original mutant.

This mutant must be scored on minimal medium at high pH (make TAP medium, omitting acetic acid and using HCl to bring pH to 8.3).

Eversole RA (1956) Biochemical Mutants of Chlamydomonas reinhardi. Amer. J. Bot. 43:404-407

Levine RP, Goodenough UW (1970) The genetics of photosynthesis and of the chloroplast in Chlamydomonas reinhardi. Annu Rev Genet. 4:397-408

• Locus: AC6
• Chromosome: 7